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Yersinia Selective Agar

Yersinia Selective Agar is used with cefsulodin and novobiocin for the isolation of Yersinia enterocolitica in a laboratory setting. Yersinia Selective Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Yersinia enterocolitica is a significant food or water borne enteric pathogen. Yersinia Selective Agar is a selective and differential medium supporting good growth of Y. enterocoliticaand some Yersinia spp. The formula is based on Cefsulodin-Irgasan-Novobiocin (CIN) Agar developed by Schiemann. In comparison with MacConkey Agar and SS Agar, Schiemann found CIN Agar provided increased inhibition of normal enteric organisms and provided improved direct recovery of Y. enterocolitica from feces. Schiemann later modified his formula to further improve growth and recovery of Y. enterocolitica.

 

Formula Liter
Enzymatic Digest of Gelatin 17 g
Enzymatic Digest of Casein 1.5 g
Enzymatic Digest of Animal Tissue 1.5 g
Yeast Extract 2 g
Mannitol 20 g
Sodium Deoxycholate 0.5 g
Sodium Cholate 0.5 g
Sodium Chloride 1 g
Sodium Pyruvate 2 g
Magnesium Sulfate 0.01 g
Neutral Red 0.03 g
Crystal Violet 0.001 g
Irgasan® 0.004 g
Agar 13.5 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Antimicrobic / 10 mL

Cefsulodin, 4 mg
Novobiocin, 2.5 mg

Directions

  1. Suspend 59.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool sterilized medium to 45 – 50°C and aseptically add 10 mL of a sterilized aqueous solution containing 4 mg of Cefsulodin and 2.5 mg of Novobiocin.

Order Details

Product # Product Description
7257A Yersinia Selective Agar, 500 g
7257T Yersinia Selective Agar, 5 kg