Acutone PPLO Broth without CV (Non-animal) is an animal-origin free formulation used for the cultivation of Mycoplasma spp. in a laboratory setting. Acutone PPLO Broth without CV (Non-animal) is not intended for use in the diagnosis of disease or other conditions in humans.
Acutone PPLO Broth without CV (Non-animal) was developed for the cultivation of Mycoplasma spp. as an alternative to PPLO Broth without CV, and is completely free of animal-origin components.
PPLO Broth without CV is prepared according to the formula described by Morton and Lecce. Acutone PPLO Broth without CV (Crystal Violet) (Non-animal) is a modification of this formula. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasmawas discovered in a case of pleuropneumonia in a cow, and referred to as "pleuropneumonia-like organism" or PPLO. Mycoplasmas belong to the class of Mollicutes "soft skin", which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 micromillimeters.
|Vegetable Infusion (Dehydrated)||12.5 g|
|Yeast Extract||3.5 g|
|Sodium Chloride||5 g|
Final pH: 7.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Sterile Serum, 300 mL
|Product #||Product Description|
|7742A||Acutone PPLO Broth without CV (Non-animal), 500 g|
|7742T||Acutone PPLO Broth without CV (Non-animal), 5 kg|