Acutone PPLO Broth without CV (Non-animal)

Acutone PPLO Broth without CV (Non-animal) is an animal-origin free formulation used for the cultivation of Mycoplasma spp. in a laboratory setting. Acutone PPLO Broth without CV (Non-animal) is not intended for use in the diagnosis of disease or other conditions in humans.

Acutone PPLO Broth without CV (Non-animal) was developed for the cultivation of Mycoplasma spp. as an alternative to PPLO Broth without CV, and is completely free of animal-origin components.

PPLO Broth without CV is prepared according to the formula described by Morton and Lecce. Acutone PPLO Broth without CV (Crystal Violet) (Non-animal) is a modification of this formula. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasmawas discovered in a case of pleuropneumonia in a cow, and referred to as "pleuropneumonia-like organism" or PPLO. Mycoplasmas belong to the class of Mollicutes "soft skin", which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 micromillimeters.


Formula Liter
Vegetable Infusion (Dehydrated) 12.5 g
Yeast Extract 3.5 g
Sodium Chloride 5 g

Final pH: 7.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


Sterile Serum, 300 mL


  1. Dissolve 21 g of the medium in 700 mL of purified water.
  2. Heat with frequent agitation and boil to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 – 50°C and aseptically add 300 mL of the appropriate sterile serum supplement.
  5. Mix thoroughly.

Order Details

Product # Product Description
7742A Acutone PPLO Broth without CV (Non-animal), 500 g
7742T Acutone PPLO Broth without CV (Non-animal), 5 kg