Acutone TPB (Non-animal) (Tryptose Phosphate Broth)

Acutone TPB (Non-animal) (Tryptose Phosphate Broth) is used for the cultivation of a wide variety of fastidious microorganisms. This product is completely free of animal-origin components in a laboratory setting. Acutone TPB (Non-animal) (Tryptose Phosphate Broth) is not intended for use in the diagnosis of disease or other conditions in humans.

Acutone TPB (Non-animal) is an infusion-free buffered medium recommended for the cultivation of fastidious, pathogenic microorganisms. Over the past decade, detection of Bovine Spongiform Encephalopathy (BSE) and Transmissible Spongiform Encephalopathies (TSE) in animals has caused great concern among pharmaceutical companies. Acutone TPB (Non-animal) fits a role in this regulated industry. This industry has a need for a nutritious, rich medium that will support the growth of fastidious microorganisms and contains no animal products.

Acutone TPB (Non-animal) is valuable in tissue culture procedures, as shown by Ginsberg, Gold, and Jordan. Acutone TPB (Non-animal) is specified for cell culture procedures.


Formula Liter
Dextrose 2 g
Sodium Chloride 5 g
Sodium Phosphate, Dibasic, Anhydrous 2.5 g
Sodium Carbonate 0.11 g
Vegetable Infusion (Dehydrated) 20 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 29.6 g of the medium in one liter of purified water.
  2. Mix thoroughly to completely dissolve the medium.
  3. Autoclave at 121°C for 10 minutes.

Order Details

Product # Product Description
7733A Acutone TPB (Non-animal) (Tryptose Phosphate Broth), 500 g