Acutone TSB (Non-animal) (Tryptic Soy Broth)

Acutone TSB (Non-animal) (Tryptic Soy Broth) is used for the cultivation of a wide variety of microorganisms. Acutone TSB (Non-animal) is completely free of animal-origin components and is in compliance with United States and European Pharmacopeia testing methodologies as stated for Tryptic Soy Broth in a laboratory setting. Acutone TSB (Non-animal) (Tryptic Soy Broth) is not intended for use in the diagnosis of disease or other conditions in humans.

Tryptic Soy Broth (TSB) is a general purpose medium that is commonly referred to as Soybean-Casein Digest Medium. Acutone TSB (Non-animal) was developed as an alternative to Tryptic Soy Broth (7164), and is completely free of animal-origin components. Over the past decade, detection of Bovine Spongiform Encephalopathy (BSE) and Transmissible Spongiform Encephalopathies (TSE) in animals has caused great concern among pharmaceutical companies. Acutone TSB (Non-animal) fits a role in this regulated industry. This industry has a need for a nutritious, rich medium that will support the growth of fastidious microorganisms and contains no animal products.

TSB is recommended for testing in cosmetics, food industry, and chosen by the USDA Animal and Plant Health Inspection Service for detecting bacteria in live vaccines. Acutone TSB (Non-animal) is an alternative to Tryptic Soy Broth.


Formula Liter
Vegetable Infusion (Dehydrated) 17 g
Enzymatic Digest of Soybean Meal 3 g
Sodium Chloride 5 g
Dipotassium Phosphate 2.5 g
Dextrose 2.5 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 30 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7729A Acutone TSB (Non-animal) (Tryptic Soy Broth), 500 g
7729B Acutone TSB (Non-animal) (Tryptic Soy Broth), 2 kg