BCYE Agar Base is used for the isolation of Legionella spp. in a laboratory setting. BCYE Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.
In 1977, McDade et al. identified Legionella pneumophila as the causative agent of Legionnaires' disease, a multisystem disease manifested primarily by pneumonia. In 1978 a new medium, F-G Agar, resulted in improved growth of L. pneumophila, a very fastidious organism. Freely et al. modified F-C Agar by substituting yeast extract as a vitamin source and replacing starch with activated charcoal, producing Charcoal Yeast Extract (CYE) Agar. In 1980, Pasculle et al. reported that CYE Agar could be improved by the addition of ACES (N-2-acetamido-2-aminoethane sulfonic acid) buffer. One year later, Edelstein further increased the sensitivity of the medium by adding the potassium salt of alpha-ketoglutaric acid.
|Yeast Extract||10 g|
|ACES Buffer||10 g|
|Charcoal, Activated||1.5 g|
|Ferric Pyrophosphate||0.25 g|
Final pH: 6.9 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Supplements / 10 mL
L-Cysteine (4%), sterile
|Product #||Product Description|
|7728A||BCYE Agar Base, 500 g|