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Bismuth Sulfite Agar

Bismuth Sulfite Agar is used for the selective isolation of Salmonella spp. in a laboratory setting. Bismuth Sulfite Agar is not intended for use in the diagnosis of disease or other conditions in humans, and conforms to FDA/BAM formulation.

Salmonellosis can result from consumption of raw, undercooked, or improperly processed foods contaminated with Salmonella. U.S. federal guidelines require various poultry products to be routinely monitored before distribution for human consumption.

Bismuth Sulfite Agar is a modification of Wilson and Blair formula. The typhoid organism grows luxuriantly on the medium, forming characteristic black colonies. Gram-positive bacteria and coliforms are inhibited on Bismuth Sulfite Agar. The inhibitory action of Bismuth Sulfite Agar permits the use of a large inoculum, increasing the possibility of recovering pathogens that may be present in small numbers. Bismuth Sulfite Agar is generally accepted for routine detection of most Salmonella spp. Bismuth Sulfite Agar is used for the isolation of S. typhiand other Salmonella spp. from food. Bismuth Sulfite Agar is a standard methods medium for industrial applications and the laboratory environment.

 

Formula Liter
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 5 g
Beef Extract 5 g
Dextrose 5 g
Disodium Phosphate 4 g
Ferrous Sulfate 0.3 g
Bismuth Sulfite Indicator 8 g
Brilliant Green 0.025 g
Agar 20 g

Final pH: 7.5 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 52 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute.
  3. Mix thoroughly to obtain a uniform suspension prior to dispensing.
  4. Freshly prepared Bismuth Sulfite Agar is very selective, therefore suitable for heavily contaminated samples. However, it is not recommended to store prepared Bismuth Sulfite Agar at 4°C for longer than 2 days; after 3 days of storage the medium may change to a green color with a reduction in selectivity of competing microorganisms and an increased potential for the inhibition of Salmonella colonies.

Order Details

Product # Product Description
7113A Bismuth Sulfite Agar, 500 g
7113T Bismuth Sulfite Agar, 5 kg