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Blood Agar Base No. 2

Blood Agar Base No. 2 is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms in a laboratory setting. Blood Agar Base No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.

Blood agar bases are typically supplemented with 5 – 10% sheep, rabbit, or horse blood for use in isolating, cultivating, and determining hemolytic reactions of fastidious pathogenic microorganisms. Without enrichment, blood agar bases can be used as general purpose media.

In 1919, Brown experimented with blood agar formulations for the effects of colony formation and hemolysis. Blood Agar Base No. 2 is a nutritionally rich medium for maximum recovery of fastidious microorganisms. Blood Agar Base media are specified in standard method procedures for food testing.

 

Formula Liter
Enzymatic Digest of Casein 7.5 g
Enzymatic Digest of Animal Tissue 7.5 g
Liver Digest 2.5 g
Yeast Extract 5 g
Sodium Chloride 5 g
Agar 12 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 39.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Prepare 5 – 10% blood agar by aseptically adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45 – 50°C.

Order Details

Product # Product Description
7266A Blood Agar Base No. 2, 500 g
7266T Blood Agar Base No. 2, 5 kg