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BMA Agar

BMA (Buffered Mug Agar) is used with LMG Agar and the ISO-GRID® and/or NEO-GRID® Membrane Filtration System for the direct enumeration of Glucuronidase-Positive Escherichia coli in a laboratory setting. BMA (Buffered Mug Agar) is not intended for use in the diagnosis of disease or other conditions in humans.

Buffered MUG Agar is formulated to take advantage of the ability of 4-methylumbelliferone to fluoresce at an alkaline pH under long wave (365 nm) ultraviolet light. The formula consists of phosphate buffered saline modified to contain 4-methylumbelliferyl-β-D-glucuronide (100 mg/L) and 1.5% (w/v) agar. The formula enables rapid detection of β-glucuronidase activity in bacterial colonies previously exposed to sodium glucuronate, an inducer of β-glucuronidase production by E. coli.

Buffered MUG Agar is recommended to be used with Lactose Monensin Glucuronate (LMG) Agar for detection and enumeration of E. coli from all foods using the ISO-GRID and/or NEO-GRID Membrane Filtration Method.

 

Formula Liter
Sodium Phosphate, Dibasic 8.23 g
Sodium Phosphate, Monobasic 1.2 g
Sodium Chloride 5 g
4-Methylumbelliferyl-β-D-Glucuronide 0.1 g
Agar 15 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 29.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
6903A BMA Agar (Buffered MUG Agar), 500 g