Brain-Heart Infusion Broth

Brain-Heart Infusion Broth is used for the cultivation of a wide variety of fastidious organisms in a laboratory setting. Brain-Heart Infusion Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Rosenow prepared a rich medium for culturing streptococci by combining dextrose broth and brain tissue. Hayden modified the original formula while working with dental pathogens. The current formula is a modification of Rosenow and Hayden, using dehydrated infusions of porcine brain and heart tissue.

Brain-Heart Infusion Broth can be supplemented with antibiotics, varying amounts of sodium chloride, yeast extract, and serum to provide a rich medium for bacteria, yeasts and pathogenic fungi. The addition of 0.1% agar can be used to lower oxygen tension, providing an atmosphere to support the growth of aerobic, microaerophilic, and obligate anaerobic microorganisms.

Brain-Heart Infusion Broth, abbreviated as BHI, is specified in many references for food and water testing.


Formula Liter
Brain Heart Infusion 17.5 g
Enzymatic Digest of Gelatin 10 g
Dextrose 2 g
Sodium Chloride 5 g
Disodium Phosphate 2.5 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 37 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7116A Brain-Heart Infusion Broth, 500 g
7116T Brain-Heart Infusion Broth, 5 kg