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Brilliant Green Agar with Sulfapyridine

Brilliant Green Agar with Sulfapyridine is used for the selective enrichment of Salmonella spp. in a laboratory setting. Brilliant Green Agar with Sulfapyridine is not intended for use in the diagnosis of disease or other conditions in humans.

Brilliant Green Agar was first described by Kristensen et al. and later modified by Kauffmann. The outstanding selectivity of this medium permits the use of moderately heavy inocula evenly distributed over the surface. The addition of sulfonamides into Brilliant Green Agar further inhibits Escherichia coli and Proteus spp. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk.

 

Formula Liter
Yeast Extract 3 g
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 5 g
Sodium Chloride 5 g
Lactose 10 g
Sucrose 10 g
Brilliant Green 0.0125 g
Phenol red 0.08 g
Sodium Sulfapyridine 1 g
Agar 20 g

Final pH: 6.9 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 59 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7299A Brilliant Green Agar with Sulfapyridine, 500 g
7299T Brilliant Green Agar with Sulfapyridine, 5 kg