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Brilliant Green Agar

Brilliant Green Agar is used for the selective isolation of Salmonella spp. in a laboratory setting. Brilliant Green Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Kristensen, Lester, and Jurgens first described the use of Brilliant Green Agar as a primary plating medium for the isolation of Salmonella. The report described the medium as useful for the differentiation of "paratyphoid B" from other intestinal Gram-negative bacilli. Kaufmann modified their formula and used Brilliant Green Agar in addition to Tetrathionate Broth for the isolation of Salmonella from stool specimens. The outstanding selectivity of this medium permits use of moderately heavy inocula, which should be evenly distributed over the surface. Brilliant Green Agar is used in the microbial limits test. Brilliant Green Agar supplemented with novobiocin is used in food testing.

 

Formula Liter
Yeast Extract 3 g
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 5 g
Sodium Chloride 5 g
Lactose 10 g
Sucrose 10 g
Brilliant Green 0.0125 g
Phenol Red 0.08 g
Agar 20 g

Final pH: 6.9 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 58 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7117A Brilliant Green Agar, 500 g
7117T Brilliant Green Agar, 5 kg