Buffered Listeria Enrichment Broth Base (BLEB) is used with supplements for selective enrichment of Listeria spp. in foods in a laboratory setting. Buffered Listeria Enrichment Broth Base (BLEB) is not intended for use in the diagnosis of disease or other conditions in humans.
Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in food industries. Evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate.
Buffered Listeria Enrichment Broth, a modification of the formula by Lovett et al. was developed after research concluded that enrichment properties can be improved by increasing the buffering capacity of the medium with the addition of disodium phosphate. BLEB Base is based upon the FDA/BAM recommendations where the medium is supplemented with selective agents after an initial 4 hour, non-selective, pre-enrichment.
|Enzymatic Digest of Casein||17 g|
|Enzymatic Digest of Soybean Meal||3 g|
|Yeast Extract||6 g|
|Sodium Chloride||5 g|
|Monpotassium Phosphate||1.35 g|
|Dipotassium Phosphate||2.5 g|
|Disodium Phosphate||9.6 g|
Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Acriflavine HCl, 4.5 mg
Nalidixic Acid, 18 mg
Cycloheximide, 22.5 mg
|Product #||Product Description|
|7675A||Buffered Listeria Enrichment Broth Base, 500 g|
|7675T||Buffered Listeria Enrichment Broth Base, 5 kg|