Buffered Listeria Enrichment Broth

Buffered Listeria Enrichment Broth is used for selective enrichment of Listeria spp. in a laboratory setting. Buffered Listeria Enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. Evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.

Buffered Listeria Enrichment Broth, a modification of the formula by Lovett et al., was developed after subsequent work concluded that enrichment properties can be improved by increasing the buffering capacity of the medium with the addition of Disodium Phosphate. Listeria spp. grow over a pH range of 5.0 – 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.


Formula Liter
Enzymatic Digest of Casein 17 g
Enzymatic Digest of Soybean Meal 3 g
Yeast Extract 6 g
Dextrose 2.5 g
Sodium Chloride 5 g
Monpotassium Phosphate 1.35 g
Dipotassium Phosphate 2.5 g
Disodium Phosphate 9.6 g
Cycloheximide 0.05 g
Nalidixic Acid 0.04 g
Acriflavin 0.015 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 47 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7579A Buffered Listeria Enrichment Broth, 500 g
7579T Buffered Listeria Enrichment Broth, 5 kg