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Campylobacter Enrichment Broth (Bolton’s)

Campylobacter Enrichment Broth (Bolton's) is used with antimicrobics for the selective enrichment of Campylobacter spp. in a laboratory setting. Campylobacter Enrichment Broth (Bolton's) is not intended for use in the diagnosis of disease or other conditions in humans.

Campylobacter spp. are microaerophilic, very small, curved, thin, Gram-negative rods. Microaerophilic organisms have a tendency to be more sensitive to toxic forms of oxygen. Campylobacter Enrichment Broth (Bolton's), along with nutritional ingredients, contains compounds which enhance the aerotolerance of microaerophilic bacteria by suppressing the toxic form of oxygen. Campylobacter Enrichment Broth (Bolton's) is recommended in food testing. Blood-Free Campylobacter Enrichment Broth, Bolton's (2X Concentration) is described by the USDA.

 

Formula Liter
Enzymatic Digest of Animal Tissue 10 g
Lactalbumin Hydrolysate 5 g
Yeast Extract 5 g
Sodium Chloride 5 g
Hemin 0.01 g
Sodium Pyruvate 0.5 g
α-Ketoglutamic Acid 1 g
Sodium Metabisulfite 0.5 g
Sodium Carbonate 0.6 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Campylobacter Supplement (Bolton), 5 mL

2.5 mL ethanol / 2.5 mL purified water

Enrichment

Lysed Horse Blood, 50 mL

Antimicrobic / 10 mL of Ethanol

Cefoperazone 20 mg
Cycloheximide 50 mg
Trimethoprim 20 mg
Vancomycin 20 mg

Directions

  1. Dissolve 27.6 g of the medium in one liter of purified water.
  2. Allow powder to soak for 10 minutes.
  3. Heat with frequent agitation to completely dissolve the medium.
  4. Autoclave at 121°C for 15 minutes.
  5. Cool medium to 45 - 50°C and if desired, aseptically add 50 mL of lysed horse blood and 2 vials of reconstituted Campylobacter Supplement (Boltons) (# 7998), OR 10 mL of ethanol (USP grade recommended)3 containing 20 mg of Cefoperazone, 50 mg of Cycloheximide, 20 mg Trimethoprim, and 20 mg Vancomycin.
  6. Campylobacter Supplement (Boltons) (# 7998) is aseptically reconstituted by adding 2.5 mL ethanol (USP grade) and 2.5 mL of sterile purified water to the vial. Gently swirl to dissolve the contents.
  7. Note: Blood-Free Campylobacter Enrichment Broth, Bolton's (2X Concentration) is described by the USDA.

Order Details

Product # Product Description
7526A Campylobacter Enrichment Broth (Bolton's), 500 g
7526T Campylobacter Enrichment Broth (Bolton's), 5 kg