Campylobacter Enrichment Broth (Bolton's) is used with antimicrobics for the selective enrichment of Campylobacter spp. in a laboratory setting. Campylobacter Enrichment Broth (Bolton's) is not intended for use in the diagnosis of disease or other conditions in humans.
Campylobacter spp. are microaerophilic, very small, curved, thin, Gram-negative rods. Microaerophilic organisms have a tendency to be more sensitive to toxic forms of oxygen. Campylobacter Enrichment Broth (Bolton's), along with nutritional ingredients, contains compounds which enhance the aerotolerance of microaerophilic bacteria by suppressing the toxic form of oxygen. Campylobacter Enrichment Broth (Bolton's) is recommended in food testing. Blood-Free Campylobacter Enrichment Broth, Bolton's (2X Concentration) is described by the USDA.
Formula | Liter |
Enzymatic Digest of Animal Tissue | 10 g |
Lactalbumin Hydrolysate | 5 g |
Yeast Extract | 5 g |
Sodium Chloride | 5 g |
Hemin | 0.01 g |
Sodium Pyruvate | 0.5 g |
α-Ketoglutamic Acid | 1 g |
Sodium Metabisulfite | 0.5 g |
Sodium Carbonate | 0.6 g |
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Campylobacter Supplement (Bolton), 5 mL
2.5 mL ethanol / 2.5 mL purified water
Enrichment
Lysed Horse Blood, 50 mL
Antimicrobic / 10 mL of Ethanol
Cefoperazone 20 mg
Cycloheximide 50 mg
Trimethoprim 20 mg
Vancomycin 20 mg
Product # | Product Description |
7526A | Campylobacter Enrichment Broth (Bolton's), 500 g |
7526T | Campylobacter Enrichment Broth (Bolton's), 5 kg |