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Clostridium difficile Agar

Clostridium difficile Agar is used with blood, Cycloserine, and Cefoxitin for the isolation of Clostridium difficile in a laboratory setting. Clostridium difficile Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Clostridium difficile has been isolated from diverse natural habitats, including soil, hay, sand, dung from various large mammals, and from dog, cat, and rodent feces.

In 1979, George et al. developed a medium called CCFA (Cycloserine-Cefoxitin-Fructose agar), based on the Egg Yolk Agar formula of McClung and Toabe with fructose replacing glucose. Clostridium difficile Agar is a modification of the original CCFA formulation.

 

Formula Liter
Enzymatic Digest of Casein 15 g
Enzymatic Digest of Animal Tissue 15 g
Pork Brain Heart Infusion Solids 10 g
Fructose 6 g
Disodium Phosphate 5 g
Monopotassium Phosphate 1 g
Magnesium Sulfate 0.1 g
Sodium Chloride 2 g
Agar 15 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplements

Horse Blood, 7%
Cycloserine, 0.5 g/L
Cefoxitin, 0.016 g/L

Directions

  1. Suspend 69 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 – 50°C and aseptically add 7% horse blood, Cycloserine (0.5 g/L) and Cefoxitin (0.016 g/L).

Order Details

Product # Product Description
7385A Clostridium difficile Agar, 500 g
7385T Clostridium difficile Agar, 5 kg