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Clostridium perfringens Agar Base

Clostridium perfringens Agar Base is used with selective and differential supplements for the recovery of Clostridium perfringens in a laboratory setting. Clostridium perfringens Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Clostridium perfringens Agar Base is a nutritious base medium used in the preparation of SFP Agar and TSC Agar. Depending upon the formula, supplements are added to increase the selectivity of the medium. Shahidi-Ferguson Perfringens (SFP) Agar is based on the formula by Shahidi and Ferguson and incorporates the supplements, Polymyxin B Sulfate and Kanamycin. Tryptose Sulphite Cycloserine (TSC) Agar is developed with the same base as SFP Agar, with the addition of Cycloserine. If desired, Egg Yolk Emulsion can be added to either formula.

 

Formula Liter
Enzymatic Digest of Casein 15 g
Enzymatic Digest of Soybean Meal 5 g
Yeast Extract 5 g
Sodium Metabisulfite 1 g
Ferric Ammonium Citrate 1 g
Agar 14 g

Final pH: 7.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement (If desired)

Egg Yolk Emulsion, 100 mL, 50% Solution

Directions

For SFP and TSC:

  1. Suspend 41 g of the medium in 900 mL of purified water. Mix thoroughly.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Cool to 45 – 50°C.
  4. Add 100 mL of Egg Yolk Emulsion, 50% (# 7982) and the desired antimicrobic supplement:
    TSC Agar: Add 10 mL of a sterile 4% solution of Cycloserine.
    SFP Agar: Add 10 mL of a sterile solution containing 3 mg of Polymyxin B Sulfate and 12 mg of Kanamycin.

For Overlay Agar:

  1. Suspend 41 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Cool to 45 - 50°C.
  4. Overlay agar does not get supplemented with Egg Yolk Emulsion. Overlay agar is held at 45 -50°C until used, and should be poured right after inoculation.

Order Details

Product # Product Description
9188A Clostridium perfringens Agar Base, 500 g