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Columbia Agar

Columbia Agar is used with or without blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Conforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Columbia Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Columbia Agar is a nutritiously rich general purpose medium developed by Ellner et al. from Columbia University. This medium contains a mixture of peptones, which provides a blend of nitrogenous compounds and amino acids to enhance growth. Columbia Agar is used for the cultivation of fastidious microorganisms and as a base for the preparation of various specialized culture media to which supplements can be added. Columbia Agar can be supplemented with 5 – 10% sheep, rabbit, or horse blood for use in isolating, cultivating, and determining hemolytic reactions of fastidious pathogenic microorganisms.

The addition of antibiotics, kanamycin or gentamicin, to Columbia Agar suppresses the growth of certain Gram-negative and Gram-positive microorganisms. Columbia Agar can provide an anaerobic environment when supplemented with growth factors, hemin, vitamin K1, and sheep blood.

Columbia Agar conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).

 

Formula Liter
Pancreatic Digest of Casein 10 g
Meat Peptic Digest 5 g
Heart Pancreatic Digest 3 g
Yeast Extract 5 g
Maize Starch 1 g
Sodium Chloride 5 g
Agar 12 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 41 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7734A Columbia Agar, 500 g
7734T Columbia Agar, 5 kg