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Columbia Blood Agar Base

Columbia Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms in a laboratory setting. Columbia Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Columbia blood agar base media are typically supplemented with 5 – 10% sheep, rabbit, or horse blood for use in isolating, cultivating and determining hemolytic reactions of fastidious pathogenic microorganisms. Without enrichment, Columbia Blood Agar Base is used as a general purpose media.

Columbia Blood Agar Base was developed after the Columbia Agar formulation described by Ellner et al. from Columbia University. Columbia (Blood Agar Base) BAB is specified in the Compendium of Methods for the Microbiological Examination of Foods.

 

Formula Liter
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 8 g
Yeast Enriched Peptone 10 g
Corn Starch 1 g
Sodium Chloride 5 g
Agar 14 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 43 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Prepare 5 – 10% blood agar by aseptically adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45 – 50°C.

Order Details

Product # Product Description
7125A Columbia Blood Agar Base, 500 g
7125T Columbia Blood Agar Base, 5 kg