Columbia Broth

Columbia Broth is used for the cultivation of a wide variety of fastidious microorganisms in a laboratory setting. Columbia Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Columbia Broth is prepared according to the formula described by Morello and Ellner. During their study, Columbia Broth was developed as a medium for blood cultures, and found superior to a commonly used broth for growth of Staphylococcus aureusE. coli, and streptococci.


Formula Liter
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 5 g
Yeast Enriched Peptone 10 g
Enzymatic Digest of Heart Muscle 3 g
Sodium Chloride 5 g
Dextrose 2.5 g
L-Cystine 0.1 g
Magnesium Sulfate 0.1 g
Ferrous Sulfate 0.02 g
Tris (hydroxymethyl) aminomethane 0.83 g
Tris (hydroxymethyl) aminomethane-HCl 2.86 g
Sodium Carbonate 0.6 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 35 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7127A Columbia Broth, 500 g
7127T Columbia Broth, 5 kg