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Cooked Meat Medium

Cooked Meat Medium is used for the cultivation of anaerobic microorganisms in a laboratory setting. Cooked Meat Medium is not intended for use in the diagnosis of disease or other conditions in humans.

In 1890, Smith used fresh unheated animal tissue for cultivating anaerobic organisms. Tarozzi confirmed Smith's findings and discovered meat broth could be heated to 104–105°C for 15 minutes without destroying nutrients. A steam sterilized emulsion of brain tissue in water was employed by von Hibler. von Hibler found organisms in cooked brain broth were less susceptible to harmful effects of toxic metabolic products than in carbohydrate serum media. Robertson substituted beef heart for brain tissue and Cooked Meat Medium is prepared according to this formula.

Cooked Meat Medium initiates growth from a small inoculum. Cooked Meat Medium is recommended in standard methods for food testing. Cooked Meat Medium provides an effective maintenance medium. This medium can be used to differentiate saccharolytic from proteolytic Clostridium spp. Saccharolytic species rapidly form acid and gas without digesting meat. Proteolytic species break down meat to amino acids and form sulfur compounds (blackening and putrid smell).

 

Formula Liter
Beef Heart 454 g
Enzymatic Digest of Animal Tissue 20 g
Dextrose 2 g
Sodium Chloride 5 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Place 1.25 g of meat granules into a test tube and add 10 mL of purified water.
  2. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7110A Cooked Meat Medium, 500 g
7110T Cooked Meat Medium, 5 kg