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DC Medium with BCIG

DC Medium with BCIG is used in the chromogenic differentiation of E. coli from other coliforms in water samples using the membrane filtration method in a laboratory setting. DC Medium with BCIG is not intended for use in the diagnosis of disease or other conditions in humans.

DC (Differential Coliform) Medium with BCIG is a selective and differential medium for the presumptive identification of E. coli. This medium is enhanced by the addition of a chromogenic agent, BCIG, 5-bromo-4-chloro-3-indolyl-β-D-glucuronide to detect glucuronidase activity. The presence of the enzyme β-D-glucuronidase differentiates most E. coli spp. from other coliforms, and is the same enzyme used in the MUG reaction. BCIG reacts slightly differently, and when released into the medium is insoluble, accumulating within the cell, imparting a blue to purple color to presumptive E. coli colonies.

 

Formula Liter
Lactose 10 g
Tryptose 10 g
Yeast Extract 3 g
Sodium Chloride 5 g
Proteose Peptone 5 g
Bile Salts 1.5 g
BCIG 0.2 g
Neutral Red 0.08 g
Agar 15 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement

Cefsulodin solution, 10 mg/mL

Directions

  1. Dissolve 49.8 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.
  4. Cool to 45 – 50°C.
  5. Aseptically add 1.2 mL of a sterile 10 mg/mL solution of Cefsulodin and mix well.

Order Details

Product # Product Description
7704A DC Medium with BCIG, 500 g
7704T DC Medium with BCIG, 5 kg