D/E Neutralizing Broth with Tween

D/E Neutralizing Broth with Tween is used for testing and neutralizing of antiseptics and disinfectants in a laboratory setting. D/E Neutralizing Broth with Tween is not intended for use in the diagnosis of disease or other conditions in humans.

D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer.

Total neutralization of disinfectants is critical. Disinfectant residues can result in a false-negative (no-growth) test. D/E Neutralizing Broth with Tween effectively neutralizes the inhibitor action of disinfectant carryover, allowing differentiation between bacteriostasis and the true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralizing Broth with Tween is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.


Formula Liter
Dextrose 10 g
Lecithin 7 g
Sodium Thiosulfate 6 g
Polysorbate 80 5 g
Enzymatic Digest of Casein 5 g
Yeast Extract 2.5 g
Sodium Bisulfite 2.5 g
Sodium Thioglycollate 1 g
Bromcresol Purple 0.02 g

Final pH: 7.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 39 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7705A D/E Neutralizing Broth with Tween, 500 g
7705T D/E Neutralizing Broth with Tween, 5 kg