D/E Neutralizing Broth

D/E Neutralizing Broth is used for testing and neutralizing of antiseptics and disinfectants in a laboratory setting. D/E Neutralizing Broth is not intended for use in the diagnosis of disease or other conditions in humans.

D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer.

Total neutralization of disinfectants is critical. Disinfectant residues can result in a false-negative (no-growth) test. D/E Neutralizing Broth effectively neutralizes the inhibitor action of disinfectant carryover, allowing differentiation between bacteriostasis and the true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralizing Broth is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.


Formula Liter
Enzymatic Digest of Casein 5 g
Yeast Extract 2.5 g
Dextrose 10 g
Sodium Thioglycollate 1 g
Sodium Thiosulfate 6 g
Sodium Bisulfite 2.5 g
Lecithin 7 g
Bromcresol Purple 0.02 g

Final pH: 7.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement / Liter

Polysorbate 80, 5 g


  1. Dissolve 34 g of the medium and 5 g of Polysorbate 80 in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Ordering Information

Product # Product Description
7562A D/E Neutralizing Broth, 500 g
7562T D/E Neutralizing Broth, 5 kg