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Dermatophyte Test Medium

Dermatophyte Test Medium is used for the selective isolation of dermatophytic fungi in a laboratory setting. Dermatophyte Test Medium is not intended for use in the diagnosis of disease or other conditions in humans.

In 1969, Taplin et al. developed this medium for the isolation and recognition of dermatophytic fungi, the causative agent of ringworm from hair, nails, and skin. Dermatophyte Test Medium is preferred for isolation and early recognition of MicrosporumTrichophyton, and Epidermophyton genera because of a distinct color change in the medium. Rapidly-growing species may produce a complete color change in the medium in 3 days. The slower-growing species will change the indicator in longer time periods. Other organisms may grow, but can be recognized as nondermatophytes by lack of a color change. A few organisms, including saprophytes, yeasts, and bacteria are capable of changing the medium from red to yellow, but are easily recognized by their distinctive colonial morphology.

 

Formula Liter
Enzymatic Digest of Soybean Meal 10 g
Dextrose 10 g
Phenol Red 0.2 g
Cycloheximide 0.5 g
Agar 20 g

Final pH: 5.5 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplements

Gentamicin, 0.1 g/L
Chlortetracycline, 0.1 g/L

Directions

  1. Suspend 40.7 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 50°C and aseptically add Gentamicin (0.1 g/L) and Chlortetracycline (0.1 g/L).

Order Details

Product # Product Description
7265A Dermatophyte Test Medium, 500 g
7265T Dermatophyte Test Medium, 5 kg