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Dichloran Glycerol (DG-18) Agar Base

Dichloran Glycerol (DG-18) Agar Base is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. Dichloran Glycerol (DG-18) Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Dichloran Glycerol Agar Base is based on the formulation by Hocking and Pitt. This medium is recommended for enumeration and isolation of yeasts and molds from dried and semi-dried foods, including fruits, spices, cereals, nuts, meat, and fish products. The highly selective composition of this medium allows for enumeration of fungal growth. In a comparative study between DG-18 Agar Base and DRBC Agar, greater recovery of two molds commonly isolated in high numbers from dried foods grew poorly on DRBC Agar.

A modification of DG-18 Agar Base, enhanced with Triton-X, is described as increasing inhibition of vigorously-spreading fungi.

 

Formula Liter
Peptone 5 g
Glucose 10 g
Monopotassium Phosphate 1 g
Magnesium Sulfate 0.5 g
Zinc Sulfate 0.01 g
Copper Sulfate 0.005 g
Dichloran 0.002 g
Chloramphenicol 0.05 g
Chlortetracycline 0.05 g
Agar 15 g

Final pH: 5.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement

Glycerol, 220 g

Directions

  1. Suspend 31.6 g of the medium and 220 g of glycerol in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. DO NOT OVERHEAT.

Order Details

Product # Product Description
7592A Dichloran Glycerol (DG-18) Agar Base, 500 g
7592T Dichloran Glycerol (DG-18) Agar Base, 5 kg