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DRBC Agar

DRBC Agar is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. DRBC Agar is not intended for use in the diagnosis of disease or other conditions in humans.

DRBC Agar is based on Dichloran Rose Bengal Chlortetracycline (DRBC) Agar formula described by King, Hocking, and Pitt. DRBC Agar conforms with APHA guidelines for the mycological examination of foods containing chloramphenicol rather than chlortetracycline as proposed by King, Hocking, and Pitt. DRBC Agar is a selective medium, supporting good growth of yeasts and molds.

 

Formula Liter
Enzymatic Digest of Animal Tissue 5 g
Glucose 10 g
Monopotassium Phosphate 1 g
Magnesium Sulfate 0.5 g
Rose Bengal 0.025 g
Dichloran 0.002 g
Chloramphenicol 0.1 g
Agar 15 g

Final pH: 5.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 31.6 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. DO NOT OVERHEAT.

Order Details

Product # Product Description
7591A DRBC Agar, 500 g
7591T` DRBC Agar, 5 kg