EE Broth, Mossel

EE Broth, Mossel is used for the cultivation and enrichment of Enterobacteriaceae in food in a laboratory setting. EE Broth, Mossel is not intended for use in the diagnosis of disease or other conditions in humans. EE Broth Mossel, conforms to Harmonized USP/EP/JP Requirements.

EE Broth, Mossel was developed by Mossel, Visser, and Cornelissen to facilitate the growth of Enterobacteriaceae. This medium contains dextrose to enhance the growth of E. coli and Salmonella spp., particularly in food samples. Nuisance organisms are suppressed by the addition of Ox Bile and Brilliant Green.

EE Broth, Mossel is used as an enrichment broth, providing a rich environment for the recovery of damaged or injured cells. Enterobacteriaceae organisms can be injured in food-processing procedures, including exposure to low temperature, sub-marginal heat, drying, radiation, preservatives, or sanitizers. The enumeration of Enterobacteriaceae is an important measure of the sanitary condition of food. Although injured cells may not form colonies on selective media, they can cause infection if ingested.

EE Broth, Mossel complies with the specifications of the Eiprodukte-Verodnung (German Egg Product Regulations) and conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).


Formula Liter
Desiccated Ox Bile 20 g
Enzymatic Digest of Gelatin 10 g
Sodium Phosphate, Dibasic 8 g
Dextrose 5 g
Potassium Phosphate, Monobasic 2 g
Brilliant Green 0.015 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 45 g of the medium in one liter of purified water.
  2. Heat at 100°C for 30 minutes to completely dissolve the medium.
  3. Cool rapidly in cold water.

Order Details

Product # Product Description
7603A EE Broth, Mossel, 500 g
7603T EE Broth, Mossel, 5 kg