Eosin Methylene Blue Agar (Holt, Harris & Teague) is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. Eosin Methylene Blue Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Eosin Methylene Blue Agar (EMB) was developed by Holt-Harris and Teague. This formula contains lactose and sucrose with two indicator dyes, Eosin Y and Methylene Blue. The use of Eosin Y and Methylene Blue as indicators produced sharp and distinct differentiation between colonies of lactose fermenting and non-fermenting organisms. Sucrose is included to detect coliforms that ferment sucrose more readily than lactose. EMB Agar is selective due to the presence of an inhibitor and differential based on the ability of some organisms to ferment carbohydrates with the absorption of an Eosin Y and Methylene Blue complex.
EMB Agar is recommended for use in examining clinical specimens for enteric pathogens.
|Enzymatic Digest of Gelatin||10 g|
|Dipotassium Phosphate||2 g|
|Eosin Y||0.4 g|
|Methylene Blue||0.065 g|
Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
|Product #||Product Description|
|7134A||Eosin Methylene Blue Agar (Holt, Harris & Teague), 500 g|