Fluid Thioglycollate Medium

Fluid Thioglycollate Medium is used for sterility testing in a laboratory setting. Conforms to Harmonized USP/EP/JP Requirements. Fluid Thioglycollate Medium is not intended for use in the diagnosis of disease or other conditions in humans.

Quastel and Stephenson found that the presence of small amounts of a compound containing an –SH group (cysteine, thioglycollic acid and glutathione) permitted "aerobic" growth of Clostridium sporogenes. Falk, Bucca and Simmons, discovered the advantages of using small quantities of agar in detecting contaminants during sterility testing. The value of a small amount of agar and a reducing substance was demonstrated by Brewer.

Fluid Thioglycollate Medium is also referred to as Thioglycollate Medium, and abbreviated FTM. Fluid Thioglycollate Medium is prepared according to the formula specified in the FDA Bacteriological Analytical Manual (BAM), conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP) requirements, and the AOAC Official Methods of Analysis for the examination of food, and sporicidal effects of disinfectants.


Formula Liter
Enzymatic Digest of Casein 15 g
Yeast Extract 5 g
Dextrose 5.5 g
L-Cystine 0.5 g
Sodium Chloride 2.5 g
Sodium Thioglycollate 0.5 g
Resazurin 0.001 g
Agar 0.75 g

Final pH: 7.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 29.8 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7137A Fluid Thioglycollate Medium, 500 g
7137T Fluid Thioglycollate Medium, 5 kg