Fraser Broth is used with Acriflavin, Nalidixic Acid, and Ferric Ammonium Citrate for the selective enrichment of Listeria spp. in a laboratory setting. Fraser Broth is not intended for use in the diagnosis of disease or other conditions in humans.
Listeria monocytogenes, described first in 1926 by Murray, Webb and Swann, is an extensive problem in food industries. Evidence from outbreaks of listeriosis indicated the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese and pate.
Fraser Broth is based on the formulation of Fraser and Sperber. This medium is used in rapid detection of Listeria from food and environmental samples. Listeria spp. grow over a pH range of 5.0 – 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria spp. is based on successful isolation of the organism, biochemical characterization, and serological confirmation.
|Enzymatic Digest of Casein||5 g|
|Enzymatic Digest of Animal Tissue||5 g|
|Beef Extract||5 g|
|Yeast Extract||5 g|
|Sodium Chloride||20 g|
|Disodium Phosphate||12 g|
|Monopotassium Phosphate||1.35 g|
|Lithium Chloride||3 g|
Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Supplement / 10 mL
Acriflavin, 25 mg
Nalidixic Acid, 20 mg
Supplement / 5 mL
Ferric Ammonium Citrate, 250 mg
|Product #||Product Description|
|7626A||Fraser Broth, 500 g|
|7626T||Fraser Broth, 5 kg|