HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.
Methods for isolating molds from cosmetic products require incubation for 5 – 7 days using traditional agar media. In 1986, Mead and O'Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O'Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.
|Enzymatic Digest of Casein||2.5 g|
|Enzymatic Digest of Animal Tissue||2.5 g|
|Yeast Extract||5 g|
|Disodium Phosphate||3.5 g|
|Monopotassium Phosphate||3.4 g|
|Ammonium Chloride||1.4 g|
|Sodium Carbonate||1 g|
|Magnesium Sulfate||0.06 g|
Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Supplement / Liter
Tween 80, 20 mL
|Product #||Product Description|
|7520A||HC Agar Base, 500 g|
|7520T||HC Agar Base, 5 kg|