KF Streptococcus Agar

KF Streptococcus Agar is used with triphenyltetrazolium chloride for the selective isolation and enumeration of fecal streptococci in a laboratory setting. KF Streptococcus Agar is not intended for use in the diagnosis of disease or other conditions in humans.

KF Streptococcus Agar was developed by Kenner, Clark, and Kabler for the detection of fecal streptococci in surface waters. KF Streptococcus Agar is currently used in detecting fecal streptococci in water, foods, and other materials through the membrane filtration technique or pour plate method. Fecal streptococci are normal inhabitants in the intestines, and the presence of fecal streptococci can be used as an indication of fecal contamination. The presence of fecal streptococci is of value in determining pollution sources because certain fecal streptococci are host-specific.

KF Streptococcus Agar is recommended in standard methods for food and water testing.


Formula Liter
Enzymatic Digest of Animal Tissue 10 g
Yeast Extract 10 g
Sodium Chloride 5 g
Sodium Glycerophosphate 10 g
Maltose 20 g
Lactose 1 g
Sodium Azide 0.4 g
Bromcresol Purple 0.015 g
Agar 20 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


1% Triphenlytetrazolium Chloride (TTC)


  1. Suspend 76.4 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Cool to 45 – 50°C.
  4. Aseptically add 10 mL of a filter sterilized solution of 1% Triphenlytetrazolium Chloride. Mix well.

Order Details

Product # Product Description
7610A KF Streptococcus Agar, 500 g
7610T KF Streptococcus Agar, 5 kg