Lactobacilli MRS Agar

Lactobacilli MRS Agar is used for the cultivation of lactobacilli in a laboratory setting. Lactobacilli MRS Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Lactobacilli MRS Agar is based on the formulations of deMan, Rogosa and Sharpe (MRS). This medium supports luxuriant growth of lactobacilli from fecal, dairy, and other sources.


Formula Liter
Enzymatic Digest of Animal Tissue 10 g
Beef Extract 10 g
Yeast Extract 5 g
Dextrose 20 g
Sodium Acetate 5 g
Polysorbate 80 1 g
Potassium Phosphate 2 g
Ammonium Citrate 2 g
Magnesium Sulfate 0.1 g
Manganese Sulfate 0.05 g
Agar 15 g

Final pH: 6.5 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 70 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7543A Lactobacilli MRS Agar, 500 g
7543T Lactobacilli MRS Agar, 5 kg