Lactobacilli MRS Broth

Lactobacilli MRS Broth is used for the cultivation of lactobacilli in a laboratory setting. Lactobacilli MRS Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Lactobacilli MRS Broth is based on the formulations of deMan, Rogosa and Sharpe (MRS). This medium supports luxuriant growth of lactobacilli from dairy and other sources.


Formula Liter
Enzymatic Digest of Animal Tissue 10 g
Beef Extract 10 g
Yeast Extract 5 g
Dextrose 20 g
Sodium Acetate 5 g
Polysorbate 80 1 g
Potassium Phosphate 2 g
Ammonium Citrate 2 g
Magnesium Sulfate 0.1 g
Manganese Sulfate 0.05 g

Final pH: 6.5 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 55 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7406A Lactobacilli MRS Broth, 500 g
7406T Lactobacilli MRS Broth, 5 kg