Lactobacillus Selective Agar Base

Lactobacillus Selective Agar Base is used for the isolation and enumeration of lactobacilli in a laboratory setting. Lactobacillus Selective Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Lactobacillus Selective Agar Base was developed by Rogosa, Mitchell, and Wiseman. This medium is used for isolation, enumeration, and identification of lactobacilli in foods. The low pH and high acetate concentrations effectively suppress other bacterial flora allowing lactobacilli to flourish.


Formula Liter
Enzymatic Digest of Casein 10 g
Yeast Extract 5 g
Monopotassium Phosphate 6 g
Ammonium Citrate 2 g
Dextrose 20 g
Sodium Acetate Hydrate 25 g
Magnesium Sulfate 0.575 g
Manganese Sulfate 0.12 g
Ferrous Sulfate 0.034 g
Polysorbate 80 1 g
Agar 15 g

Final pH: 5.5 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


Glacial Acetic Acid, 1.32 mL


  1. Suspend 84 g of the medium in one liter of purified water. Mix thoroughly.
  2. Add 1.32 mL of glacial acetic acid.
  3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  4. Avoid overheating. DO NOT AUTOCLAVE.

Order Details

Product # Product Description
7234A Lactobacillus Selective Agar Base, 500 g
7234T Lactobacillus Selective Agar Base, 5 kg