Lactose Broth

Lactose Broth is used for the cultivation of Salmonella and coliform bacteria from food, dairy, and water products in a laboratory setting. Lactose Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Lactose Broth is frequently used as a pre-enrichment medium when testing foods and dairy products for Salmonella spp. In dried or processed foods, Salmonella species may be sublethally injured and in low numbers. The presence of other bacteria as well as components of the food sample may hinder growth and recovery of Salmonella. Pre-enrichment in a nonselective medium such as Lactose Broth allows for repair of cell damage, dilutes toxic or inhibitory substances, and provides a nutritional advantage to Salmonella over other bacteria. Lactose Broth is widely used and is included in many procedures for testing foods, dairy products and other materials.

Lactose Broth is also used for the detection of coliform organisms in water, dairy products, and other materials.


Formula Liter
Enzymatic Digest of Gelatin 5 g
Beef Extract 3 g
Lactose 5 g

Final pH: 6.9 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 13 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Distribute into test tubes containing Durham tubes.
  4. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7141A Lactose Broth, 500 g
7141T Lactose Broth, 5 kg