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Lauryl Sulfate Broth

Lauryl Sulfate Broth is used for the detection of coliform bacteria in water and wastewater in a laboratory setting. Lauryl Sulfate Broth is not intended for use in the diagnosis of disease or other conditions in humans.

The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriaceae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products, and other materials.

Lauryl Sulfate Broth, also referred to as Lauryl Tryptose Broth, is prepared according to the formula of Mallmann and Darby. During their investigation, Sodium Lauryl Sulfate produced the best results for inhibition of organisms other than coliforms. Lauryl Sulfate Broth, abbreviated as LSB, is used in the presumptive phase of the Standard Total Coliform Fermentation Technique in the examination of water, and coliform detection of foods.

 

Formula Liter
Enzymatic Digest of Casein 20 g
Lactose 5 g
Sodium Chloride 5 g
Monopotassium Phosphate 2.75 g
Disodium Phosphate 2.75 g
Sodium Lauryl Sulfate 0.1 g

Final pH: 6.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 35.6 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Prepared double strength broth for evaluating 10 mL samples. Distribute into tests containing inverted Durham tubes.
  4. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7142A Lauryl Sulfate Broth, 500 g
7142T Lauryl Sulfate Broth, 5 kg