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LB Agar, Lennox

LB Agar, Lennox is used in molecular genetic studies in a laboratory setting. LB Agar, Lennox is not intended for use in the diagnosis of disease or other conditions in humans.

LB Agar is nutritionally rich, developed by Lennox for growth and maintenance of pure cultures of recombinant strains of E. coli. These strains are generally derived from E. coli K12, which are deficient in B vitamin production. This strain of E. coli has been further modified through specific mutation to create an auxotrophic strain that is not capable of growth on nutritionally deficient media. LB Agar provides all nutritional requirements of these organisms. LB Agar contains half the sodium chloride level of the Miller formulation of LB Agar, allowing the researcher to select the optimal salt concentration for a specific strain.

 

Formula Liter
Enzymatic Digest of Casein 10 g
Yeast Extract 5 g
Sodium Chloride 5 g
Agar 12 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 32 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7289A LB Agar, Lennox, 500 g
7289T LB Agar, Lennox, 5 kg