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LB Agar, Miller

LB Agar, Miller is used in molecular genetic studies in a laboratory setting. LB Agar, Miller is not intended for use in the diagnosis of disease or other conditions in humans.

LB Agar, Miller, a nutritionally rich medium designed for growth of pure cultures of recombinant strains, is based on the formulation described by Miller. E. coli is grown to late log phase in LB Medium. Some plasmid vectors replicate to a high copy number and do not require selective amplification. Some vectors do not replicate so freely, and need to be selectively amplified. Chloramphenicol may be added to inhibit host synthesis and prevent replication of the bacterial chromosome.

LB Agar, Miller contains 10 g/L of sodium chloride, different from the levels in Lennox formulations. This allows the researcher to select the optimal salt concentration for a specific strain. The medium may be aseptically supplemented with glucose.

 

Formula Liter
Enzymatic Digest of Casein 10 g
Yeast Extrac 5 g
Sodium Chloride 10 g
Agar 12 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 37 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7213A LB Agar, Miller, 500 g
7213T LB Agar, Miller, 5 kg