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Listeria Enrichment Broth

Listeria Enrichment Broth is used for selective enrichment of Listeria spp. in a laboratory setting. Listeria Enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Listeria Enrichment Broth is based on the formula developed by Lovett et al. in which Tryptic Soy Broth is supplemented with Yeast Extract for optimum growth. Listeria spp. grow over a pH range of 5.0 – 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.

 

Formula Liter
Enzymatic Digest of Casein 17 g
Enzymatic Digest of Soybean Meal 3 g
Yeast Extract 6 g
Dextrose 2.5 g
Sodium Chloride 5 g
Dipotassium Phosphate 2.5 g
Cyclohexamide 0.05 g
Acriflavin 0.015 g
Nalidixic Acid 0.04 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 36.1 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7398A Listeria Enrichment Broth, 500 g
7398T Listeria Enrichment Broth, 5 kg