M-Broth is used for the cultivation of Salmonella spp. in a laboratory setting. M-Broth is not intended for use in the diagnosis of disease or other conditions in humans.

M-Broth is prepared according to the formula of Sperber and Diebel, and contains the nutrients necessary for good growth and flagella development of Salmonella. M-Broth is used for cultivating Salmonella in foods and feeds by the accelerated enrichment serology (ES) procedure. M-Broth conforms to standard procedures recommended in food testing and monoclonal and polyclonal enzyme immunoassays (EIA).


Formula Liter
Enzymatic Digest of Casein 12.5 g
Yeast Extract 5 g
D-Mannose 2 g
Sodium Citrate 5 g
Sodium Chloride 5 g
Potassium Phosphate 5 g
Manganese Chloride 0.14 g
Magnesium Sulfate 0.8 g
Ferrous Sulfate 0.04 g
Polysorbate 80 0.75 g

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 36.2 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7296A M-Broth, 500 g
7296T M-Broth, 5 kg