m-FC Broth

m-FC Broth is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment.

Many standard method membrane filtration procedures recommend m-FC media for testing water. The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 ± 0.5°C in several procedures. The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.


Formula Liter
Enzymatic Digest of Casein 10 g
Enzymatic Digest of Animal Tissue 5 g
Yeast Extract 3 g
Sodium Chloride 5 g
Lactose 12.5 g
Bile Salts 1.5 g
Aniline Blue 0.1 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


1% Rosolic Acid, 1 mL


m-FC Broth

  1. Dissolve 3.7 g of the medium in 100 mL of purified water containing 1 mL of 1% rosolic acid in 0.2 N NaOH.
  2. Heat with frequent agitation to boiling to completely dissolve the medium.
  3. Cool to room temperature.

Rosolic Acid

  1. Dissolve 1 g in 100 mL of 0.2 N NaOH to prepare a 1% solution.

Order Details

Product # Product Description
7396A m-FC Broth, 500 g