m-FC Broth is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Broth is not intended for use in the diagnosis of disease or other conditions in humans.
Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment.
Many standard method membrane filtration procedures recommend m-FC media for testing water. The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 ± 0.5°C in several procedures. The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.
|Enzymatic Digest of Casein||10 g|
|Enzymatic Digest of Animal Tissue||5 g|
|Yeast Extract||3 g|
|Sodium Chloride||5 g|
|Bile Salts||1.5 g|
|Aniline Blue||0.1 g|
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
1% Rosolic Acid, 1 mL
|Product #||Product Description|
|7396A||m-FC Broth, 500 g|