m-TEC Agar is used with urea for the isolation and enumeration of thermotolerant Escherichia coli from water using the membrane filtration technique in a laboratory setting. m-TEC Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Escherichia coli is used as an indicator of fecal pollution in water. Several tests are available for enumerating E. coli based on its ability to grow at elevated temperatures and indole production. The membrane filter procedure is recognized in Standard Methods as an alternate test procedure. m-TEC is an abbreviation for membrane thermotolerant E. coli.
In 1981, Dufour et al. developed a simple and accurate membrane filter technique for rapid enumeration of E. coli. In this study, the researchers were able to quantitate E. coli on m-TEC Agar within 24 hours without requiring subculture and identification of isolates. Dufour et al. recovered E. coli from marine, estuarine, and fresh water samples.
|Enzymatic Digest of Animal Tissue||5 g|
|Yeast Extract||3 g|
|Sodium Chloride||7.5 g|
|Potassium Phosphate||4.3 g|
|Sodium Lauryl Sulfate||0.2 g|
|Sodium Deoxycholate||0.1 g|
|Bromcresol Purple||0.08 g|
|Bromphenol Red||0.08 g|
Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Note: Other methods may recommend an alternative pH. Prepare substrate according to recommended guidelines.
|Product #||Product Description|
|7421A||m-TEC Agar, 500 g|
|7421B||m-TEC Agar, 2 kg|
|7421T||m-TEC Agar, 5 kg|