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m-TGE Broth

m-TGE Broth is used for the determination of bacterial counts using membrane filtration in a laboratory setting. m-TGE Broth is not intended for use in the diagnosis of disease or other conditions in humans.

m-TGE is an abbreviation for membrane Tryptone Glucose Extract. In the 1930's, Bower and Hucker developed a medium for detecting bacteria in milk and other dairy products. Prickett used a glucose agar containing tryptone to study thermophilic bacteria in milk. This medium is known as Yeast Dextrose Agar. In 1948, the American Public Health Association (APHA) adopted Tryptone Glucose Extract Agar for use in testing milk and dairy products. Currently, APHA specifies Tryptone Glucose Extract Agar for the heterotrophic plate count procedure in testing bottled water.

m-TGE Broth is a nonselective nutrient medium for the determination of bacterial counts by the membrane filtration method. This broth has the same formulation as Tryptone Glucose Extract Agar, except the agar has been omitted and ingredients are at twice the concentration.

 

Formula Liter
Enzymatic Digest of Casein 10 g
Beef Extract 6 g
Dextrose 2 g

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 18 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7451A m-TGE Broth, 500 g
7451T m-TGE Broth, 5 kg