MacConkey Agar with Sorbitol is used for the isolation of pathogenic Escherichia coli in a laboratory setting. MacConkey Agar with Sorbitol is not intended for use in the diagnosis of disease or other conditions in humans.
MacConkey Agar with Sorbitol is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7. Serotype O157 has been implicated in serious foodborne diseases.
MacConkey Agar with Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar with Sorbitol is recommended for food testing.
| Formula | Liter |
| Enzymatic Digest of Gelatin | 17 g |
| Enzymatic Digest of Casein | 1.5 g |
| Enzymatic Digest of Animal Tissue | 1.5 g |
| Sorbitol | 10 g |
| Bile Salts Mixture | 1.5 g |
| Sodium Chloride | 5 g |
| Neutral Red | 0.03 g |
| Crystal Violet | 0.001 g |
| Agar | 13.5 g |
Final pH: 7.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
| Product # | Product Description |
| 7320A | MacConkey Agar with Sorbitol, 500 g |
| 7320T | MacConkey Agar with Sorbitol, 5 kg |