Mannitol Salt Agar is used for the isolation of staphylococci in a laboratory setting. Mannitol Salt Agar is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to Harmonized USP/EP/JP Requirements.
Chapman formulated Mannitol Salt Agar to isolate staphylococci by inhibiting growth of most other bacteria with a high salt concentration. Chapman added 7.5% Sodium Chloride to Phenol Red Mannitol Agar, and noted pathogenic strains of staphylococci (coagulase-positive staphylococci) grew luxuriantly and produced yellow colonies with yellow zones. Non-pathogenic staphylococci produced small red colonies with no color change to the surrounding medium.
Mannitol Salt Agar is highly selective, and specimens from heavily contaminated sources may be streaked onto this medium without danger of overgrowth. Mannitol Salt Agar is recommended for isolating pathogenic staphylococci from specimens, cosmetics, and microbial limit tests.
|Enzymatic Digest of Casein||5 g|
|Enzymatic Digest of Animal Tissue||5 g|
|Beef Extract||1 g|
|Sodium Chloride||75 g|
|Phenol Red||0.025 g|
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
|Product #||Product Description|
|7143A||Mannitol Salt Agar, 500 g|
|7143T||Mannitol Salt Agar, 5 kg|