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Mannitol Salt Agar

Mannitol Salt Agar is used for the isolation of staphylococci in a laboratory setting. Mannitol Salt Agar is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to Harmonized USP/EP/JP Requirements.

Chapman formulated Mannitol Salt Agar to isolate staphylococci by inhibiting growth of most other bacteria with a high salt concentration. Chapman added 7.5% Sodium Chloride to Phenol Red Mannitol Agar, and noted pathogenic strains of staphylococci (coagulase-positive staphylococci) grew luxuriantly and produced yellow colonies with yellow zones. Non-pathogenic staphylococci produced small red colonies with no color change to the surrounding medium.

Mannitol Salt Agar is highly selective, and specimens from heavily contaminated sources may be streaked onto this medium without danger of overgrowth. Mannitol Salt Agar is recommended for isolating pathogenic staphylococci from specimens, cosmetics, and microbial limit tests.

 

Formula Liter
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 5 g
Beef Extract 1 g
D-Mannitol 10 g
Sodium Chloride 75 g
Phenol Red 0.025 g
Agar 15 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 111 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7143A Mannitol Salt Agar, 500 g
7143T Mannitol Salt Agar, 5 kg