Middlebrook 7H11 Agar is used with glycerol and OADC Enrichment for the cultivation of Mycobacterium spp. in a laboratory setting. Middlebrook 7H11 Agar is not intended for use in the diagnosis of disease or other conditions in humans.
There are two types of solid culture media for the primary isolation of mycobacteria, coagulated egg as a base (Lowenstein formulations) and an agar base (Middlebrook formulations). The use of agar-based media for primary isolation of mycobacteria have the following significant advantages:
Middlebrook 7H11 Agar is a modification of Middlebrook 7H10 Agar Special as recommended by Cohn, Waggoner, and McClately. Cohn et al. added an enzymatic digest of casein and found organism growth was stimulated for fastidious strains of Mycobacterium tuberculosis.
|Enzymatic Digest of Casein||1 g|
|Disodium Phosphate||1.5 g|
|Monopotassium Phosphate||1.5 g|
|Ammonium Sulfate||0.5 g|
|Monosodium Glutamate||0.5 g|
|Sodium Citrate||0.4 g|
|Ferric Ammonium Citrate||0.04 g|
|Magnesium Sulfate||0.05 g|
|Copper Sulfate||0.001 g|
|Zinc Sulfate||0.001 g|
|Malachite Green||0.00025 g|
Final pH: 6.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Glycerol, 5 mL
OADC Enrichment, 100 mL
|Product #||Product Description|
|7244A||Middlebrook 7H11 Agar, 500 g|
|7244T||Middlebrook 7H11 Agar, 5 kg|