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Mueller Hinton Agar

Mueller Hinton Agar is used in antimicrobial susceptibility testing by the disk diffusion method. This formula conforms to Clinical and Laboratory Standard Institute (CLSI), formerly National Committee for Clinical Laboratory Standards (NCCLS).

Mueller Hinton Agar is based on the formula recommended by Mueller and Hinton for the primary isolation of Neisseria spp. Mueller and Hinton selected pea meal extract agar as a simple transparent medium containing heat stable ingredients. During their modification, starch replaced the growth-promoting properties of pea extract, acting as a "protective colloid" against toxic substances.

Bauer, Kirby, Sherris and Tuck recommended Mueller Hinton Agar for performing antibiotic susceptibility tests using a single disk of high concentration. This unsupplemented medium has been selected by the Clinical and Laboratory Standard Institute (CLSI) for several reasons. This medium is low in sulfonamide, trimethoprim and tetracycline inhibitors, and provides satisfactory growth of most non-fastidious pathogens along with demonstrating batch-to-batch reproducibility.

Mueller Hinton Agar is often abbreviated as M-H Agar, and complies with requirements of the World Health Organization. Mueller Hinton Agar is specified in FDA Bacteriological Analytical Manual for food testing, and procedures commonly performed on aerobic and facultatively anaerobic bacteria. A variety of supplements can be added to Mueller Hinton Agar, including 5% defibrinated sheep or horse blood, 1% growth supplement and 2% sodium chloride.

 

Formula Liter
Beef Extract 2 g
Acid Hydrolsate of Casein 17.5 g
Starch 1.5 g
Agar 17 g

Final pH: 7.3 ± 0.1 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 38 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Cool to room temperature.
  4. OPTIONAL: Supplement as appropriate. Pour cooled Mueller Hinton Agar into sterile Petri dishes on a level, horizontal surface to give uniform depth. Allow to cool to room temperature.
  5. Check prepared Mueller Hinton Agar to ensure the final pH is 7.3 ± 0.1 at 25°C.

Order Details

Product # Product Description
7101A Mueller Hinton Agar, 500 g
7101T Mueller Hinton Agar, 5 kg