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MYP Agar base

MYP Agar Base is used with supplements for the selective and differential enumeration of Bacillus cereus from foods. Conforms to FDA / BAM formulation in a laboratory setting. MYP Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

MYP Agar Base is also referred to as Mannitol-Egg Yolk-Polymyxin Agar. Mossel et al developed MYP Agar for the isolation of Bacillus cereus in foods. This medium differentiates B. cereus from other bacteria based on resistance to Polymyxin B, lack of Mannitol fermentation, and presence of Lecithinase.

Food poisoning caused by Bacillus cereus can occur when the organism is allowed to proliferate. This can occur when foods are prepared and held without sufficient refrigeration. B. cereus can be found on vegetables, raw and processed foods, and in nature. Outbreaks of foodborne illness have been associated with boiled and cooked rice, cooked meats, soups, custards, and cooked vegetables. Two types of illness have been attributed to the consumption of foods contaminated with B. cereus. The first is characterized by abdominal pain and diarrhea, with an incubation period of 4 – 16 hours and symptoms that last for 12 – 24 hours. The second involves an acute attack of nausea and vomiting, occurring within 1 – 5 hours after consumption. MYP Agar Base is recommended for standard methods in food testing.

 

Formula Liter
Beef Extract 1 g
Peptone 10 g
D-Mannitol 10 g
Sodium Chloride 10 g
Phenol red 25 mg
Agar 15 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplements

Egg Yolk Emulsion, 100 mL
Polymyxin B (50,000 IU), 5 mL

Directions

  1. Suspend 23 g of the medium in 450 mL of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 – 50°C.
  5. Aseptically supplement with 25 mL Egg Yolk Emulsion (7982) and 5 mL of a 50,000 IU Polymyxin B solution (7997).

Order Details

Product # Product Description
7741A MYP Agar Base, 500 g
7741T MYP Agar Base, 5 kg