Nutrient Agar

Nutrient Agar is used for the cultivation of a wide variety of microorganisms in a laboratory setting. Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans.

In the early 1900's, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Agar continues to be a widely used general purpose medium for growing nonfastidious microorganisms. If required, enrichments can be added to this medium. Nutrient Agar, modified by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG), is used for fluorogenic detection of Escherichia coli.

Nutrient Agar meets APHA and Association of Official Analytical Chemists (AOAC) standard methods. Nutrient Agar is specified in many standard methods procedures for the examination of food, dairy products, water, and other materials.


Formula Liter
Enzymatic Digest of Gelatin 5 g
Beef Extract 3 g
Agar 15 g

Final pH: 6.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 23 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7145A Nutrient Agar, 500 g
7145T Nutrient Agar, 5 kg